This surveillance report presents information on first-time diagnoses from all thirteen provinces and territories between 2014 and 2023, and provides robust evidence for the planning, evaluation, and implementation of HIV prevention and care programs and education. This report also presents information on first-time HIV diagnoses and previously diagnosed HIV by province and territory for 2020 to 2023 for the planning of effective treatment programs and monitoring Canada’s progress toward our 95-95-95 targets.